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Screening and identification of non-inflammatory specific protein markers in Wilms' tumor tissues

Junjie Zhang1, Fei Guo1, Lei Wang1

  • 1Department of Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, 450052, Henan, China.

Archives of Biochemistry and Biophysics
|September 25, 2019
PubMed

Insights

This study identified profilin-1 as a specific protein marker for childhood Wilms

Area of Science:

  • Pediatric Oncology
  • Proteomics
  • Biomarker Discovery

Background:

  • Wilms' tumor is a common pediatric malignancy requiring early diagnosis.
  • Previous research utilized proteomics to find serum protein markers in Wilms' tumor patients.
  • Identifying specific tumor markers is crucial for diagnosis and treatment.

Purpose of the Study:

  • To identify specific protein markers for Wilms' tumor.
  • To compare proteomic profiles of Wilms' tumor tissues and sera with normal kidney tissues and systemic inflammatory response syndrome (SIRS) controls.

Main Methods:

  • Proteomic analysis using Surface-Enhanced Laser Desorption Ionization Time-of-Flight Mass Spectrometry (SELDI-TOF-MS).
  • Protein purification via Sodium Dodecylsulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE).
  • Protein identification using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight/Time-of-Flight (MALDI-TOF/TOF).
  • Validation of expression using Real-Time Polymerase Chain Reaction (RT-PCR) and Enzyme-Linked Immunosorbent Assay (ELISA).

Main Results:

  • SELDI-TOF-MS identified a specific protein marker (m/z 8350) in Wilms' tumor.
  • The identified protein was confirmed as profilin-1.
  • Profilin-1 expression was validated in Wilms' tumor samples.

Conclusions:

  • Profilin-1 is a potential protein marker for Wilms' tumor.
  • The employed proteomic methods are feasible for identifying tumor-specific markers.
  • This finding supports improved diagnostic strategies for Wilms' tumor.

Keywords:
Profilin-1Protein markerProteomicsWilms' tumor tissues

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