Related Experiment Video

Updated: Jan 19, 2026

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
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Changes in Protein Glycosylation as a Result of Aptamer Interactions with Cancer Cells

Anna Drabik1, Joanna Ner-Kluza1, Kinga Hartman1

  • 1Department of Biochemistry and Neurobiology, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Krakow, 30-059, Poland.

Proteomics. Clinical Applications
|September 25, 2019
PubMed
Abstract

Insights

Researchers identified specific proteins, kinesin-like protein (KI13B) and proliferating cell nuclear antigen (PCNA), that interact with aptamers in breast cancer cells. This finding advances aptamer-based cancer therapy and biomarker discovery.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Aberrant protein glycosylation is crucial in cancer progression and treatment resistance.
  • Aptamers offer therapeutic potential in oncology by targeting specific molecular mechanisms.
  • Understanding aptamer-target interactions is key for developing novel cancer therapies.

Purpose of the Study:

  • To investigate glycoproteome changes in breast cancer cells upon interaction with aptamers.
  • To identify molecular targets of aptamer-mediated therapeutic effects in breast cancer.
  • To explore aptamers as a tool for biomedical applications in cancer treatment.

Main Methods:

  • Studying the interaction between specific aptamer sequences and breast cancer cells.
  • Analyzing glycan expression on cell surface proteins.
  • Identifying proteins that bind to aptamers and display N-glycan epitopes.

Main Results:

  • Two proteins, kinesin-like protein (KI13B) and proliferating cell nuclear antigen (PCNA), were identified.
  • These proteins exhibit N-glycan epitopes after conjugation with aptamer sequences.
  • This highlights specific molecular targets for aptamer-based breast cancer interventions.

Conclusions:

  • Aptamers can be utilized as alternatives to antibodies in various biomedical applications, including cancer therapy.
  • The identified proteins (KI13B and PCNA) are potential targets for therapeutic aptamers in breast cancer.
  • This research contributes to the development of aptamer-based diagnostics and therapeutics for cancer.
Keywords:
KIF13BPCNAaptamersbreast cancerglycoproteome

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