Prolonged Biodegradation and Improved Mechanical Stability of Collagen via Vapor-Phase Ti Stitching for Long-Term...

Collagen, one of the most popular biomedical materials, exhibits rapid biodegradation accompanied by a notable decrease of mechanical stability in the human body. This is a key challenge for its use in large-sized tissue regeneration, which takes a long time. In order to resolve this problem, we introduced vapor-phase titanium (Ti) derivatives into the interchain regions in collagen via TiO₂ atomic layer deposition (ALD), which has been widely used for thin-film deposition. The introduced Ti simultaneously enhanced both the tensile strength (∼384.45 MPa) and Young's modulus (∼1.56 GPa) by approximately 29 and 26% compared to the pristine commercial collagen membrane. In vitro tests demonstrated that approximately 31% of Ti-infiltrated collagen is retained after 4 weeks, whereas the pristine commercial collagen rapidly degrades by up to 90% within 1 week. The in vivo biodegradation rate was greatly improved and inversely proportional to the number of TiO₂ ALD cycles. Moreover, bone mineralization, which is observed during the late stage of bone healing, appeared only in the Ti-infiltrated collagen. We believe that our simple vapor-phase treatment method could be widely used with xenograft materials, which typically require adequate biodegradation rates and stable mechanical properties.