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Related Experiment Video

Updated: Jan 19, 2026

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology
09:45

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology

Published on: November 14, 2025

566

High-Throughput Automated Microscopy of Circulating Tumor Cells.

Carlos Aguilar-Avelar1, Brenda Soto-García1, Diana Aráiz-Hernández1

  • 1Delee Corp., Mountain View, CA, 94041, USA.

Scientific Reports
|September 26, 2019
PubMed
Summary
This summary is machine-generated.

Automated microscopy enhances cancer cell analysis. This new system offers operator-free, robust detection of circulating tumor cells (CTCs) for improved cancer diagnosis and monitoring.

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Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment

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Area of Science:

  • Biomedical Engineering
  • Cancer Research
  • Microscopy Technology

Background:

  • Circulating tumor cells (CTCs) show promise as biomarkers for cancer management.
  • Current CTC detection methods face challenges due to equipment variability and operator error, limiting clinical adoption.

Purpose of the Study:

  • To develop a fully automated, high-throughput fluorescence microscope for imaging and classifying cancer cells.
  • To overcome limitations of existing methods for CTC analysis.

Main Methods:

  • Design and construction of a novel automated fluorescence microscope system.
  • Utilizing machine vision for automated imaging and classification of immunostained cancer cells.
  • Comparison of the automated system with state-of-the-art microscopy equipment.

Main Results:

  • The automated microscope system successfully performed high-throughput imaging and classification of cancer cells.
  • The machine vision-based approach demonstrated excellent agreement with conventional microscopy.
  • The system enables operator-free analysis of cancer cells.

Conclusions:

  • The developed automated fluorescence microscope offers a robust and operator-free solution for cancer cell analysis.
  • This technology has the potential to standardize cancer cell detection in clinical practice.
  • Facilitates reliable cancer diagnosis, prognosis, and monitoring through improved CTC analysis.