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Updated: Jan 19, 2026

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
Published on: April 7, 2014
Quantitative Phase and Intensity Microscopy Using Snapshot White Light Wavefront Sensing
Congli Wang1, Qiang Fu1, Xiong Dun1
1Visual Computing Center, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia.
Abstract:
Phase imaging techniques are an invaluable tool in microscopy for quickly examining thin transparent specimens. Existing methods are limited to either simple and inexpensive methods that produce only qualitative phase information (e.g. phase contrast microscopy, DIC), or significantly more elaborate and expensive quantitative methods. Here we demonstrate a low-cost, easy to implement microscopy setup for quantitative imaging of phase and bright field amplitude using collimated white light illumination.
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