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A Microfluidic Device for Studying Multiple Distinct Strains
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Cell motion and recovery in a two-stream microfluidic device.

Clara Mata, Ellen Longmire1, David McKenna2

  • 1Aerospace Engineering and Mechanics, University of Minnesota, 110 Union Street SE, Minneapolis, MN 55455, USA.

Microfluidics and Nanofluidics
|September 26, 2019
PubMed
Summary

This study demonstrates a microfluidic device for efficient cryoprotective agent removal from cell suspensions. The device achieves high cell recovery and viability, outperforming conventional methods.

Keywords:
Biological cellCell processingCryopreservationLaminar channel flowMicrofluidics

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Cryoprotective agents (CPAs) are essential for cell cryopreservation but require efficient removal post-thaw.
  • Conventional cell washing methods can be time-consuming and lead to cell loss.

Purpose of the Study:

  • To evaluate the performance of a two-stream microfluidic device for extracting CPAs from cell suspensions.
  • To determine the impact of flow rate and cell volume fraction (CVF) on cell viability and recovery.

Main Methods:

  • Jurkat cells in 10% dimethylsulfoxide were flowed with phosphate-buffered saline wash streams in a microfluidic device.
  • Cell viability and recovery were measured across varying cell stream velocities (3.6–8.5 mm/s) and CVFs (up to 20%).
  • Experiments were conducted in single-stage and multi-stage configurations.

Main Results:

  • High cell viability (>90%) was observed, indicating no significant cell damage.
  • Cell recovery reached 90-100% at velocities ≥6 mm/s and CVFs up to 20% in single-stage devices.
  • Multi-stage devices achieved 90-100% recovery after a critical device population time (5x average residence time).

Conclusions:

  • The microfluidic device effectively removes CPAs with high cell recovery and viability.
  • The device offers a significant improvement over conventional cell washing techniques.
  • Optimized flow rates and CVFs are crucial for maximizing recovery in microfluidic cell processing.