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Discriminating normal and inflammatory models by viscosity changes with a mitochondria-targetable fluorescent probe.
Weishan Wang1, Yong Liu, Jie Niu
1Institute of Fluorescent Probes for Biological Imaging, School of Materials Science and Engineering, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong 250022, P. R. China. weiyinglin2013@163.com.
The Analyst
|September 26, 2019
Summary
Researchers developed a fluorescent probe (EIMV) to detect changes in intracellular viscosity, aiding in the early identification of diseases like cancer and inflammation in biological and medical studies.
Area of Science:
- Biomedical Engineering
- Molecular Biology
- Cell Biology
Background:
- Intracellular viscosity is linked to critical diseases including diabetes, neurodegenerative disorders, and cancer.
- Monitoring cellular viscosity is crucial for understanding disease progression and developing diagnostic tools.
Purpose of the Study:
- To develop a novel mitochondria-targetable fluorescent probe for detecting intracellular viscosity variations.
- To validate the probe's efficacy in distinguishing between normal and diseased biological models.
Main Methods:
- Synthesis and characterization of the EIMV (Enhanced Intracellular Microviscosity) fluorescent probe.
- Evaluation of EIMV's photophysical properties, cytotoxicity, and biocompatibility.
- Application of EIMV to differentiate normal and cancer cells, and to assess viscosity changes in zebrafish and mice models.
Main Results:
- EIMV demonstrated high photostability, low cytotoxicity, red emission, and good biocompatibility.
- The probe successfully identified viscosity differences in normal versus cancer cells.
- EIMV effectively detected viscosity variations in zebrafish and mice, correlating with normal and inflammatory states.
Conclusions:
- The EIMV probe offers a sensitive and reliable method for monitoring intracellular viscosity.
- This tool facilitates the study of disease-related viscosity changes in biological and medical research.
- EIMV holds potential for early disease detection and understanding cellular pathophysiology.
