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High-efficiency CRISPR/Cas-based editing of Phalaenopsis orchid MADS genes

Chii-Gong Tong1, Fu-Hui Wu1, Yu-Hsuan Yuan1

  • 1Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan.

Plant Biotechnology Journal
|September 26, 2019
PubMed
Abstract

No abstract available in PubMed .

Keywords:
agrobacterium-mediated transformationgene familyprotospacer-adjacent motiftransformation strategy

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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