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Updated: Jan 19, 2026

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography HPLC
Published on: March 15, 2017
Retention behavior of ginsenosides in a sulfo-based high performance liquid chromatography column
Boquan Qu1, Lina Zhang1, Shaoyan Wang1
1School of Chemical and Engineering, University of Science and Technology Liaoning, Anshan, Liaoning 114051, P.R. China.
Abstract:
We herein report the use of a sulfo-based column and hydrophilic interaction chromatography (HILIC) to separate 14 ginsenosides, namely Rb1, Rb2, Rb3, Rc, Rd, Rf, Re, Rg1, Rg2, Rg3, Rh1, Rh2, F2, and C-K. In addition to its rapid and efficient ability to separate these ginsenosides, the sulfo-based column exhibited a good relationship between the ginsenoside capacity factor (k') and molecular weight (Mw) and a strict elution order corresponding to the polarity (P) of the ginsenosides, as confirmed by thin layer chromatography.
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