Related Experiment Video
Updated: Jan 19, 2026
Preparation of Polypentafluorophenyl acrylate Functionalized SiO2 Beads for Protein Purification
Published on: November 19, 2018
A supramolecular hydrogel to boost the production of antibodies for phosphorylated proteins
Youzhi Wang1, Xinxin Li2, Yiming Zhang2
1College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Design, Nankai University, Tianjin 300071, P. R. China. yangzm@nankai.edu.cn chwling@nankai.edu.cn and Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, State Key Laboratory of Medicinal Chemical Biology, and Collaborative Innovation Center of Chemical Science and Engineering, Nankai University, Tianjin 300071, P. R. China.
Antibodies are widely used both in clinical practice and in research. However, the development of methods to increase the ratio of antibodies to recognize phosphorylated proteins remains challenging. In this study, we report a novel and useful method for the efficient production of antibodies for phosphorylated proteins. Based on our previously developed vaccine adjuvant Nap-GDFDFDY, we prepared hydrogels by the Ca2+-induced self-assembly of a phosphorylated peptide gelator Nap-GDFDFpDY. The hydrogel could protect phosphorylated antigens from being dephosphorylated by endogenous phosphatase, thus selectively increasing the ratio of the antibodies for phosphorylated proteins. Our study provides a useful strategy for the production of antibodies to recognize proteins with specific posttranslational modifications.
Antibodies are widely used both in clinical practice and in research. However, the development of methods to increase the ratio of antibodies to recognize phosphorylated proteins remains challenging. In this study, we report a novel and useful method for the efficient production of antibodies for phosphorylated proteins. Based on our previously developed vaccine adjuvant Nap-GDFDFDY, we prepared hydrogels by the Ca2+-induced self-assembly of a phosphorylated peptide gelator Nap-GDFDFpDY. The hydrogel could protect phosphorylated antigens from being dephosphorylated by endogenous phosphatase, thus selectively increasing the ratio of the antibodies for phosphorylated proteins. Our study provides a useful strategy for the production of antibodies to recognize proteins with specific posttranslational modifications.
