Related Experiment Video

Updated: Jan 19, 2026

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate
07:31

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate

Published on: May 3, 2021

4.2K

CSN5 controls the growth of osteosarcoma via modulating the EGFR/PI3K/Akt axis

Zongmiao Wan1, Sheng Huang1, Fengbo Mo1

  • 1Department of Orthopedic Surgery, First Affiliated Hospital of Nanchang University, Nanchang, 330006, China.

Experimental Cell Research
|September 28, 2019
PubMed

Insights

Constitutive photomorphogenesis 9 signalosome subunit 5 (CSN5) promotes osteosarcoma growth by stabilizing EGFR, activating the PI3K/Akt pathway. CSN5 overexpression correlates with poor prognosis, suggesting it as a potential diagnostic and therapeutic target for osteosarcoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • The constitutive photomorphogenesis 9 signalosome (CSN) subunit CSN5 is implicated in various cancers.
  • The role of CSN5 in osteosarcoma (OS) pathogenesis and its clinical significance remain largely unknown.

Purpose of the Study:

  • To investigate the biological functions and clinical relevance of CSN5 in osteosarcoma.
  • To elucidate the underlying molecular mechanisms of CSN5's action in OS.

Main Methods:

  • Comparative analysis of CSN5 expression in OS tumors versus normal bone tissues.
  • In vitro and in vivo studies assessing the impact of CSN5 overexpression and silencing on OS cell growth and tumorigenicity.
  • Investigation of the involvement of the PI3K/Akt signaling pathway and epidermal growth factor receptor (EGFR) in CSN5-mediated effects.

Main Results:

  • CSN5 is overexpressed in OS tumors and associated with malignant phenotypes and poor patient prognosis.
  • High CSN5 expression promotes OS cell growth and tumorigenicity, while CSN5 silencing inhibits these processes.
  • CSN5 activates the PI3K/Akt pathway, and its oncogenic effects are dependent on EGFR stabilization via reduced ubiquitination.

Conclusions:

  • CSN5 plays a crucial role in osteosarcoma development and progression.
  • CSN5's regulation of EGFR stability and subsequent activation of the PI3K/Akt pathway are key mechanisms in OS pathogenesis.
  • CSN5 represents a promising diagnostic biomarker and therapeutic target for osteosarcoma.
Keywords:
CSN5EGFROsteosarcomaPI3K/Akt signaling pathwayUbiquitination

More Related Videos

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

7.6K
Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

11.2K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.6K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
7.7K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.4K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.1K