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Updated: Jan 19, 2026
A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
Published on: November 23, 2015
Wavelength-scanning surface plasmon resonance microscopy: A novel tool for real time sensing of cell-substrate
Youjun Zeng1, Jie Zhou1, Xueliang Wang1
1College of Physics and Optoelectronics Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen Key Laboratory of Sensor Technology, Shenzhen University, Shenzhen, 518060, China.
Abstract:
This paper, for the first time, presents a wavelength-scanning surface plasmon resonance microscope (WS-SPRM) as a label-free biosensor capable of measuring cell-substrate interaction. The approach utilized a liquid crystal tunable filter (LCTF) as a fast and flexible wavelength-scanning device that can implement a wavelength-scanning and SPR imaging cycle within 1 s. The system was verified by monitoring the dynamics of cellular processes including cell detachment and electroporation of individual cells. It was found that the WS-SPRM presented better performance than the intensity-based SPRM (I-SPRM) in the imaging of cell adhesion. The results also indicated that the WS-SPRM exhibited a larger dynamic range in monitoring cell electroporation than that of I-SPRM. In summary, the developed WS-SPRM in this study provides a promising technique for real-time monitoring of cell-substrate interaction.
