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Updated: Aug 4, 2026

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells
Published on: July 22, 2013
[Redox regulation in ATP synthesis]
Abstract:
Evidence is considered which points to changes of redox potential of the redox-centres in mitochondria during energization, to high sensitivity of ATP-synthetase to redox agents. Examples of ATP-syntheses in model systems stimulated with an electron are discussed. This stimulation is so efficient that it permits weakening of the bond between phosphorus atom and extremely bad leaving group O- in inorganic phosphate-phosphorylating agent in ATP synthesis during oxidative phosphorylation. The sum of these data suggests that function of the redox-centres found in the coupling site may be the accumulation of the intermediate inducing ADP and Phinorg interactions. The electron pool may serve as an intermediate. Thus the redoxcentre function in the coupling site accepting and accumulating the electrons during energization may be compared with chlorophyll function in photosynthesis. Change of redox potential of redox-centres at energization (by protonation, for example) initiates electron transfer in ATP-synthetase, which by the formation of highly reactive-free radical of ADP provides the occurrence of endergonic reaction of ATP synthesis, i.e. storage of energy as a chemical bond.
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