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In phage lambda, cos is a recombinator in the red pathway.

F W Stahl, I Kobayashi, M M Stahl

    Journal of Molecular Biology
    |January 20, 1985
    PubMed
    Summary

    Bacteriophage lambda

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    Area of Science:

    • Molecular Biology
    • Genetics
    • Microbiology

    Background:

    • Bacteriophage lambda DNA replication and packaging involve specific sites like cos.
    • Red-mediated recombination is a key process in bacteriophage lambda genetic exchange.
    • The role of the cos site in Red-mediated recombination has been previously studied.

    Purpose of the Study:

    • To investigate the mechanism of cos-stimulated, Red-mediated genetic exchange in bacteriophage lambda.
    • To determine the role of the cos site as a double-strand cut site in recombination.
    • To challenge the predictions of the break-copy model regarding cos function.

    Main Methods:

    • Analyzing recombination frequencies in lambda crosses with varying cos site configurations.
    • Examining the genetic products of recombination to determine the fate of the cos site.
    • Comparing results with existing models of genetic exchange.

    Main Results:

    • High Red-mediated recombination occurs near the cos site, even with only one functional parent cos.
    • Recombination results in the loss of the stimulating cos site in the product (cos-).
    • The cos site functions as a double-strand cut site, stimulating exchange.

    Conclusions:

    • A detailed model for cos-stimulated, Red-mediated genetic exchange is proposed.
    • The cos site's role in the Red pathway differs from its role in the RecBC pathway.
    • This mechanism highlights the importance of specific DNA sites in bacteriophage genetic processes.

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