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Updated: Jan 6, 2026

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Integrative analysis reveals CRHBP inhibits renal cell carcinoma progression by regulating inflammation and apoptosis

Kang Yang1, Yusha Xiao2, Tao Xu1

  • 1Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China.

Cancer Gene Therapy
|October 2, 2019
PubMed

Insights

Discovering novel biomarkers for kidney cancer is crucial. This study identifies CRHBP as a potential therapeutic target for clear cell renal cell carcinoma (ccRCC), showing its role in suppressing tumor growth and improving patient survival.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Renal cell carcinoma (RCC) often exhibits drug resistance and poor prognosis.
  • The underlying molecular mechanisms driving RCC progression remain incompletely understood.

Purpose of the Study:

  • To identify potential biomarkers and therapeutic targets for clear cell renal cell carcinoma (ccRCC).
  • To elucidate the functional role of identified biomarkers in ccRCC development and progression.

Main Methods:

  • Integrative analysis of The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets.
  • Validation of candidate biomarkers across four independent cohorts, clinical samples, and RCC cell lines.
  • In vitro and in vivo experiments to assess the functional impact of biomarker overexpression.
  • Western blot analysis to investigate the molecular pathways involved.

Main Results:

  • Three genes (CRHBP, RAB25, PSAT1) were identified as potential ccRCC biomarkers.
  • Decreased CRHBP expression in ccRCC tumors correlated with significantly poorer patient survival.
  • Overexpression of CRHBP suppressed tumor cell proliferation, migration, invasion, and apoptosis.
  • CRHBP functions by upregulating the NF-κB and p53-mediated mitochondrial apoptotic pathway.

Conclusions:

  • CRHBP is a potential prognostic biomarker for ccRCC.
  • CRHBP acts as a tumor suppressor in ccRCC.
  • CRHBP represents a promising therapeutic target for ccRCC treatment.

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