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Related Experiment Video

Updated: Jan 6, 2026

Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture
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Spatiotemporal regulation of multipotency during prostate development.

Elisavet Tika1, Marielle Ousset1, Anne Dannau1

  • 1Laboratory of Stem Cells and Cancer, Université Libre de Bruxelles (ULB), Brussels 1070, Belgium.

Development (Cambridge, England)
|October 3, 2019
PubMed
Summary

Prostate development involves stem cells (SCs) switching from multipotent to unipotent. This study reveals how basal SCs orchestrate prostate branching and structure formation during postnatal growth.

Keywords:
Basal multipotencyCell fateLineage tracingMouseProstate developmentStem cells

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Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Urology

Background:

  • The prostate gland arises from a branched glandular epithelium comprising basal cells (BCs) and luminal cells (LCs).
  • Prostate development and maintenance in adult mice rely on multipotent and unipotent stem cells (SCs), but their fate regulation is unclear.

Purpose of the Study:

  • To investigate the cellular dynamics and spatiotemporal regulation of stem cell fate during postnatal prostate development in mice.
  • To characterize the transition from multipotency to unipotency in prostate stem cells.

Main Methods:

  • Lineage tracing
  • Whole-tissue imaging
  • Clonal analysis
  • Proliferation kinetics

Main Results:

  • Multipotent basal stem cells (SCs) initially distribute throughout the epithelium.
  • SCs progressively restrict to the distal duct tips, guiding prostate branching and structure formation.
  • Pubertal development is driven by unipotent lineage-restricted SCs.

Conclusions:

  • This study elucidates the spatiotemporal regulation of stem cell fate during prostate development.
  • It reveals a critical switch from multipotency to unipotency in basal stem cells, orchestrating gland morphogenesis.