Simultaneous single-cell profiling of chromatin, transcriptome and surface markers with OneCell CUT&Tag captures...

Area of Science:

• Single-cell biology
• Epigenetics
• Transcriptomics

Background:

• Simultaneous mapping of chromatin states and transcriptomes in rare cell populations is difficult.
• Existing methods often require large cell numbers and struggle with multi-layer accuracy within single cells.

Purpose of the Study:

• Introduce OneCell CUT&Tag for matched multi-omic profiling from single cells.
• Enable high-resolution epigenome, transcriptome, and surface marker quantification.
• Facilitate the study of rare cells in development and disease.

Main Methods:

• Developed OneCell CUT&Tag, a novel single-cell multi-omics method.
• Achieved matched high-resolution epigenome, full-transcriptome, and surface marker quantification.
• Required minimal input, as low as one cell, without metacell aggregation.

Main Results:

• Uncovered epigenomic priming in mammary gland basal cells.
• Captured dynamics of basal-to-luminal cell transdifferentiation, showing asynchronous remodeling.
• Revealed the role of H3K27me3 in zygotic expression programs.

Conclusions:

• OneCell CUT&Tag effectively integrates multiple molecular layers within individual cells.
• This method advances the study of cellular identity and state in rare biological samples.
• It provides a powerful tool for understanding development and disease mechanisms.