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Abnormal fertility, acrosome formation, IFT20 expression and localization in conditional Gmap210 knockout mice
Zhenyu Wang1,2, Yuqin Shi1, Suheng Ma1,2
1School of Medicine, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Wuhan University of Science and Technology, Wuhan, China.
American Journal of Physiology. Cell Physiology
|October 3, 2019
Summary
GMAP210 (TRIP11) is crucial for male fertility in mice. Disruption of GMAP210 impairs acrosome biogenesis and sperm head formation, leading to reduced fertility and sperm counts.
Area of Science:
- Reproductive Biology
- Cell Biology
- Genetics
Background:
- GMAP210 (TRIP11) is a cis-Golgi protein involved in intraflagellar transport.
- IFT20 is essential for male fertility and spermiogenesis.
- The role of GMAP210 in male reproduction is not fully understood.
Purpose of the Study:
- To investigate the function of GMAP210 in male fertility and spermatogenesis in mice.
- To determine the impact of GMAP210 disruption on sperm morphology and acrosome formation.
Main Methods:
- Generated floxed Gmap210 mice crossed with Stra8-iCre mice for targeted gene disruption.
- Performed histological and electron microscopy analyses of testes and sperm.
- Assessed sperm counts, fertility, and expression/localization of key proteins (IFT20, acrosomal proteins).
Main Results:
- Gmap210 mutant mice exhibited significantly reduced sperm counts and fertility.
- Sperm from mutants showed abnormally shaped heads, defects in acrosome biogenesis, and altered mitochondrial sheath formation.
- Expression and localization of IFT20 and other acrosomal proteins were significantly affected in mutants.
Conclusions:
- GMAP210 is essential for proper acrosome biogenesis and mitochondrial sheath formation during spermiogenesis.
- GMAP210 plays a critical role in regulating the expression and localization of IFT20 and other acrosomal proteins.
- GMAP210 is indispensable for maintaining male fertility in mice.
