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Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
Published on: June 8, 2019
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Detection and Characterization of Circulating Tumor Cells by Quantitative Real-Time PCR
Francesca Salvianti1, Filomena Costanza1, Gemma Sonnati1
1Department of Experimental and Clinical Biomedical Sciences "Mario Serio", University of Florence, Florence, Italy.
Methods in Molecular Biology (Clifton, N.J.)
|October 4, 2019
Abstract:
We propose two different approaches involving the use of quantitative real-time PCR for the detection or analysis of circulating tumor cells. In one case cells are indirectly identified through the expression of a marker mRNA, while in the other one cells are enriched by size prior to be submitted to mutational analysis for a specific target. Both methods have been successfully applied to the study of circulating melanoma cells.

