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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles
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Single-Cell Biodetection by Upconverting Microspinners.

Elisa Ortiz-Rivero1, Katarzyna Prorok2, Michal Skowickł3

  • 1Fluorescence Imaging Group, Departamento de Física de Materiales, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049, Madrid, Spain.

Small (Weinheim an Der Bergstrasse, Germany)
|October 5, 2019
PubMed
Summary
This summary is machine-generated.

Upconverting spinners, acting as luminescent micro-rotors, precisely detect single-cell and single-bacteria attachment by monitoring rotation speed. This technology also enables simultaneous thermal sensing and control during biodetection events.

Keywords:
bacteriacandida celloptical trappingspinnerupconversion

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Optical Physics

Background:

  • Upconverting nanoparticles (UCNPs) enable all-optical studies via near-infrared light manipulation.
  • UCNPs exhibit optical birefringence, functioning as light-driven luminescent spinners.
  • The biomedical applications of UCNP spinners remain largely unexplored.

Purpose of the Study:

  • To demonstrate the utility of upconverting spinners for detecting single-cell and single-bacteria attachment.
  • To investigate the physical principles governing spinner deceleration upon particle attachment.
  • To integrate simultaneous thermal sensing and control into the biodetection process.

Main Methods:

  • Utilizing near-infrared light for optical tweezing and manipulation of individual upconverting spinners.
  • Real-time monitoring of spinner rotation velocity to detect attachment events.
  • Harnessing upconversion emission for simultaneous thermal sensing and control.

Main Results:

  • Accurate and specific detection of single-cell and single-bacteria attachment events was achieved.
  • The physical mechanisms linking single-attachment events to angular deceleration were elucidated.
  • Simultaneous thermal sensing and control were successfully demonstrated during attachment detection.

Conclusions:

  • Upconverting spinners offer a novel platform for high-sensitivity, real-time biodetection.
  • This technology facilitates the development of fast and cost-effective single-cell and single-bacteria detection systems.
  • The dual functionality of detection and thermal management highlights the versatility of UCNP spinners in biomedicine.