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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Optical Trapping of Nanoparticles
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Sensing with (One or Many) Upconverting Nanoparticles.

Fernando E Maturi1,2, Erving Ximendes1,2, Antonio Benayas1,2,3

  • 1Nanomaterials for Bioimaging Group, Departamento de Física de Materiales, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049 Madrid, Spain.

Accounts of Chemical Research
|April 30, 2026
PubMed
Summary
This summary is machine-generated.

Lanthanide-doped upconverting nanoparticles (UCNPs) offer unique intracellular thermometry and single-particle manipulation capabilities. Their performance in biological environments and optical traps depends heavily on surrounding conditions, requiring further study for reliable nanoscale sensing.

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Area of Science:

  • Nanotechnology and Materials Science
  • Biophysics and Chemical Biology

Background:

  • Lanthanide-doped upconverting nanoparticles (UCNPs) convert near-infrared (NIR) light to visible emission, enabling applications in biological sensing and single-particle studies.
  • UCNPs are practical for intracellular thermometry due to their narrow emission lines, photobleaching resistance, and thermal coupling, measurable with standard microscopy.
  • However, the complex intracellular environment can alter UCNP emission properties, affecting thermometry reliability.

Purpose of the Study:

  • To investigate the stability and reliability of UCNP thermometry within living cells.
  • To analyze the balance between nanoparticle confinement and laser-induced heating in optical trapping experiments.
  • To understand the influence of the surrounding environment on UCNP optical properties for improved sensing and manipulation.

Main Methods:

  • Utilized UCNPs for intracellular thermometry measurements under varying cytoplasmic conditions.
  • Employed optical trapping techniques to study single UCNP behavior, including spectral changes and rotational dynamics.
  • Investigated the impact of dopant concentration and laser power on trapping stability and thermal load.

Main Results:

  • Intracellular chemical complexity (pH, ions, viscosity, crowding) can significantly alter UCNP emission, impacting thermometric accuracy.
  • Optical trapping of UCNPs is challenged by modest forces and laser-induced heating, necessitating careful balancing of confinement and thermal effects.
  • Single-particle measurements reveal insights into light-matter interactions and mechanical properties not observable in ensemble studies.

Conclusions:

  • UCNPs show promise for nanoscale temperature and mechanical measurements but require careful consideration of environmental influences for quantitative applications.
  • Optimizing UCNP performance in optical traps involves managing the trade-off between nanoparticle confinement and laser-induced heating.
  • Further research into UCNP surface chemistry and environmental interactions is crucial for developing robust intracellular sensing and manipulation tools.