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Bacterial conjugation is a mechanism of horizontal gene transfer that enables the exchange of genetic material between bacterial cells through direct contact. This process is facilitated by a donor cell carrying a conjugative plasmid, which encodes genes necessary for pilus formation, DNA replication, and transfer. The conjugative plasmid plays a central role in initiating and executing the transfer of genetic material.The tra region of the conjugative plasmid encodes proteins responsible for...
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Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach
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Measuring Plasmid Conjugation Using Antibiotic Selection.

Jorge Rodriguez-Grande1, Raul Fernandez-Lopez2

  • 1Instituto de Biomedicina y Biotecnología de Cantabria, Universidad de Cantabria-Consejo Superior de Investigaciones Científicas, Santander, Cantabria, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|October 5, 2019
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Summary
This summary is machine-generated.

Antibiotic resistance genes on plasmids spread through bacterial conjugation. This study details classical laboratory methods for quantifying this plasmid transmission, applicable to most Proteobacteria and adaptable to other bacteria.

Keywords:
Antibiotic resistanceBacterial conjugationPlasmid conjugation

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Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Plasmid conjugation is a primary mechanism for the spread of antibiotic resistance.
  • Naturally occurring plasmids frequently harbor antibiotic resistance genes, contributing to antimicrobial resistance.
  • Understanding plasmid transmission is crucial for combating antibiotic resistance.

Purpose of the Study:

  • To describe established laboratory methods for quantifying plasmid conjugation.
  • To provide a reliable protocol for assessing the transmission of antibiotic resistance determinants.
  • To offer adaptable methods for various bacterial species and plasmid types.

Main Methods:

  • Utilizing classical methods based on antibiotic resistance determinants.
  • Quantifying plasmid conjugation under controlled laboratory conditions.
  • Applying techniques suitable for most plasmid incompatibility groups within Proteobacteria.

Main Results:

  • The described methods effectively quantify plasmid conjugation.
  • The protocols are robust for assessing the spread of antibiotic resistance genes.
  • The methods demonstrate applicability across diverse Proteobacteria.

Conclusions:

  • Classical methods provide a reliable means to quantify plasmid conjugation.
  • These techniques are essential for studying the dissemination of antibiotic resistance.
  • The described protocols are adaptable for broader microbiological research.