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Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform
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Preparation of Nanostructured Lipid Drug Delivery Particles Using Microfluidic Mixing.

Linda Hong1, Yao-Da Dong2, Ben J Boyd1

  • 1ARC Centre of Excellence in Convergent Bio-Nano Science and Technology and Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC, Australia.

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|October 5, 2019
PubMed
Summary
This summary is machine-generated.

Microfluidics offers a reproducible method for preparing cubosomes, which are ordered lipid particles. This technique provides control over particle size and structure, overcoming limitations of high-energy preparation methods.

Keywords:
Cubosomeglyceryl monooleatelipid drug deliverymicrofluidicsphytantriolsmall angle X-ray scatteringsolvent dilution.

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Biomedical Engineering

Background:

  • Cubosomes are highly ordered, self-assembled lipid particles with internal liquid crystalline structures, analogous to liposomes.
  • They are explored as stimuli-responsive delivery vehicles, but traditional high-energy preparation methods like sonication limit their applications.
  • Developing low-energy, controlled preparation techniques is crucial for advancing cubosome technology.

Purpose of the Study:

  • To investigate the impact of microfluidic preparation on the particle size distribution and internal structure of cubosomes.
  • To compare the microfluidic preparation of cubosomes using two distinct lipid systems: phytantriol and glyceryl monooleate (GMO).
  • To assess the influence of microfluidic parameters on cubosome characteristics.

Main Methods:

  • Utilized a commercial microfluidic system to prepare cubosomes from phytantriol and GMO lipid systems.
  • Investigated the effects of varying relative flow rates, total flow rate, and temperature on cubosome formation.
  • Characterized particle size distribution using dynamic light scattering (DLS) and internal structure using small-angle X-ray scattering (SAXS).

Main Results:

  • Phytantriol-based cubosomes demonstrated robustness across various processing conditions.
  • GMO-based cubosomes exhibited sensitivity to composition, reflecting challenges in their preparation via other methods.
  • Microfluidic processing influenced the particle size and internal liquid crystalline structure of both lipid systems.

Conclusions:

  • Microfluidics provides a reproducible and versatile platform for preparing complex lipid particle dispersions, including cubosomes.
  • This method offers advantages over traditional high-energy techniques, enabling better control over cubosome properties.
  • The findings highlight microfluidics as a promising approach for tailoring cubosome characteristics for specific delivery applications.