Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

125I-labelled human interferons alpha, beta and gamma: comparative receptor-binding data.

G Merlin, E Falcoff, M Aguet

    The Journal of General Virology
    |May 1, 1985
    PubMed
    Summary

    Human interferons (IFNs) alpha-2, beta, and gamma bind to distinct cell receptors. IFN-gamma does not compete with IFN-alpha/beta, indicating separate receptor systems for these crucial immune signaling molecules.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    In vitro propagation of the gum arabic tree (Acacia Senegal (L.) Willd.) 1. Developing a rapid method for producing plants.

    Plant cell reports·2013
    Same author

    Sludge drying reed beds: full- and pilot-scale study for activated sludge treatment.

    Water science and technology : a journal of the International Association on Water Pollution Research·2009
    Same author

    Treatment of septage in sludge drying reed beds: a case study on pilot-scale beds.

    Water science and technology : a journal of the International Association on Water Pollution Research·2009
    Same author

    Reduction by sonication of excess sludge production in a conventional activated sludge system: continuous flow and lab-scale reactor.

    Environmental technology·2009
    Same author

    Removal of PAHs from laboratory columns simulating the humus upper layer of vertical flow constructed wetlands.

    Chemosphere·2008
    Same author

    Study of pyrene biodegradation capacity in two types of solid media.

    The Science of the total environment·2007

    Area of Science:

    • Immunology
    • Cell Biology
    • Molecular Biology

    Background:

    • Interferons (IFNs) are critical cytokines involved in immune responses and cellular regulation.
    • Different IFN subtypes (alpha, beta, gamma) are known to mediate diverse biological functions.
    • Understanding IFN receptor interactions is key to deciphering their signaling pathways.

    Purpose of the Study:

    • To directly compare the binding characteristics of human recombinant DNA interferons (IFNs) alpha-2, beta, and gamma.
    • To investigate potential receptor sharing or distinctness among IFN-alpha/beta and IFN-gamma.
    • To elucidate the cellular receptor systems utilized by different IFN subtypes.

    Main Methods:

    • Radioligand binding assays using 125I-labelled human recombinant IFNs (alpha-2, beta, gamma).

    Related Experiment Videos

  • Competition binding studies were performed on various human lymphoid cells and embryonic fibroblasts.
  • Quantification of binding constants and receptor site numbers for each IFN subtype.

    • Main Results:

    • All three IFNs (alpha-2, beta, gamma) exhibited binding constants in the range of 10(-10) to 10(-9) M.
    • IFN-gamma did not compete with IFN-alpha 2 or IFN-beta for binding, suggesting distinct receptors.
    • IFN-alpha 2 and IFN-beta demonstrated competitive binding, consistent with shared receptors.
    • The number of binding sites for IFN-gamma varied relative to IFN-alpha 2 and IFN-beta depending on the cell type.

    Conclusions:

    • The receptor system for IFN-gamma is demonstrably unrelated to the IFN-alpha/beta receptor system.
    • These findings support a model of distinct receptor engagement for different type I and type II interferons.
    • Differential expression of IFN receptors across cell types may influence cellular responses to specific interferons.