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Systemic ß adrenergic stimulation/ sympathetic nerve system stimulation influences intraocular RAS through cAMP in

Joana Raquel Martins1, Nadine Reichhart2, Nobert Kociok2

  • 1Experimental Ophthalmology, Eye Hospital, University Medical Center Regensburg, Regensburg, Germany.

Experimental Eye Research
|October 8, 2019
PubMed
Summary

Systemic beta-adrenergic stimulation increases renin expression in the retinal pigment epithelium (RPE) via the beta-adrenergic system, impacting retinal renin-angiotensin system (RAS) activity and potentially RPE

Keywords:
Angiotensin IIIsoproterenolLosartanOcular RASReninRetinal pigment epitheliumcAMPß adrenergic receptors

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Area of Science:

  • Ophthalmology
  • Neuroscience
  • Endocrinology

Background:

  • The retina possesses a local renin-angiotensin system (RAS) distinct from the systemic circulation.
  • Retinal RAS plays roles in both normal retinal function and pathological conditions like diabetic retinopathy.
  • The retinal pigment epithelium (RPE) may influence intraretinal RAS activity in response to systemic changes.

Purpose of the Study:

  • To investigate if systemic beta-adrenergic stimulation modulates renin expression in the RPE.
  • To explore the role of the beta-adrenergic system as a regulator of the retinal RAS.

Main Methods:

  • In vivo studies using mice to assess beta-adrenergic receptor expression in RPE.
  • Analysis of renin expression in RPE following systemic isoproterenol infusion.
  • In vitro studies using polarized porcine RPE to examine renin gene expression under various stimuli.

Main Results:

  • Mouse RPE expresses beta-adrenergic receptor subtypes 1 and 2.
  • Systemic isoproterenol administration increased renin expression specifically in the RPE, an effect blocked by losartan.
  • In vitro, cAMP stimulation, but not angiotensin-2, enhanced renin promoter activity in RPE cells.

Conclusions:

  • The beta-adrenergic system is identified as a novel regulator of the retinal RAS.
  • The RPE acts as a key modulator of retinal RAS, influenced by systemic beta-adrenergic activity.
  • This finding has implications for understanding retinal function and pathologies involving the RAS.