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Dexmedetomidine Attenuates Lung Injury in Obstructive Jaundice Rats Through PI3K/Akt/HIF-1α Signaling Pathway
Luanyuan Shi1, Chunyan Guo1, Yaying Xie1
1Department of Anesthesiology, The Affiliated Hospital Inner Mongolia Medical University, Hohhot, Inner Mongolia, China.
Background:
Obstructive jaundice (OJ) could seriously affect the biochemical and immune functions in the body. Up to now, there are still poor evidences about the role of dexmedetomidine in OJ.
Methods:
Male Wistar rats were divided into four groups: Sham operation group (Sham group, n = 10), obstructive jaundice group (OJ group, n = 10), OJ + dexmedetomidine group (D group, n = 10), OJ+ dexmedetomidine +LY294002 group (DL group, n = 10). The expressions of phospho-Akt(p-Akt), HIF-1α(hypoxia inducible factor-1α), Akt mRNA, HIF-1αmRNA, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in different groups were evaluated.
Results:
The lung injury score was decreased in OJ group compared with that in DL group. The expression of the HIF-1α protein, p-Akt protein, Akt mRNA, and HIF-1α mRNA in OJ group were significantly increased (p <0.05) (vs. S group). Meanwhile, these protein molecular were significantly higher in the D group than that in OJ group (p <0.05). However, the mRNA expressions of these molecular in OJ group were significantly lower than that in D group (p <0.05). The concentrations of IL-6 and TNF-α in OJ group and D group was significantly up-regulated compared with that in S group (p <0.05). Meanwhile, IL-6 and TNF-α concentrations were significantly up-regulated in DL group compared with that in group D (p <0.05). The concentrations of IL-6 and TNF-α were significantly decreased in the D group and DL group compared with that in OJ group (p <0.05).
Conclusion:
Dexmedetomidine can attenuate lung injury in obstructive jaundice rats through PI3K/Akt/HIF-1α signaling pathway. Meanwhile, dexmedetomidine can reduce the concentrations of IL-6 and TNF-α in obstructive jaundice rats.
