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Canine dendritic cells from peripheral blood and lymph nodes
Veterinary Immunology and Immunopathology
|April 1, 1985
Summary
Researchers identified canine dendritic cells in blood and lymph nodes. These cells function as accessory cells for lymphocyte responses and stimulators in mixed leukocyte cultures.
Area of Science:
- Immunology
- Cell Biology
Background:
- Dendritic cells (DCs) are crucial immune regulators.
- Canine DC characterization and function remain incompletely understood.
Purpose of the Study:
- To identify and characterize canine dendritic cells from peripheral blood and lymph nodes.
- To evaluate their accessory and stimulatory functions in immune responses.
Main Methods:
- Canine dendritic cells were isolated using density gradient centrifugation on bovine plasma albumin (BPA).
- Cellular function was assessed via 3H-thymidine incorporation assays and mixed leukocyte cultures (MLC).
- Cells were characterized by morphology, adherence, density, and post-irradiation viability.
Main Results:
- Canine dendritic cells were successfully isolated as a low-density (LD) fraction.
- Enrichment with dendritic cells enhanced lymphocyte responses to neuraminidase-galactose oxidase (NGO).
- Dendritic cells exhibited higher stimulatory activity than peripheral blood mononuclear cells (PBMC) in MLC.
Conclusions:
- Canine dendritic cells were identified in peripheral blood and lymph nodes for the first time.
- Isolated canine dendritic cells are non-adherent, low-density, and functionally viable after irradiation.
- These cells act as accessory cells in NGO-stimulated lymphocyte proliferation and as stimulators in MLC.
