Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mechanism of co-transcriptional cap snatching by influenza polymerase.

Nature·2026

Same author

Publisher Correction: Promoter-proximal RNA polymerase II termination regulates transcription during human cell type transition.

Nature structural & molecular biology·2025

Same author

Structural basis of SARS-CoV-2 polymerase inhibition by nonnucleoside inhibitor HeE1-2Tyr.

Proceedings of the National Academy of Sciences of the United States of America·2025

Same author

Promoter-proximal RNA polymerase II termination regulates transcription during human cell type transition.

Nature structural & molecular biology·2025

Same author

Mechanism of polyadenylation-independent RNA polymerase II termination.

Nature structural & molecular biology·2024

Same author

The discovery and structural basis of two distinct state-dependent inhibitors of BamA.

Nature communications·2024

Same journal

RNAbpFlow: base pair-augmented SE(3) flow matching for conditional RNA 3D structure generation.

Nature methods·2026

Same journal

Spatio-DARLIN enables robust and efficient in situ lineage tracing in mice at single-cell resolution.

Nature methods·2026

Same journal

EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.

Nature methods·2026

Same journal

Cloud-based microscope enables live neuroimaging for 24 h and beyond with worldwide access.

Nature methods·2026

Same journal

Deep molecular profiling in three dimensions.

Nature methods·2026

Same journal

3D pathology-guided microdissection.

Nature methods·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 6, 2026

Subnanometer-resolution Structural Determination of Hemagglutinin from Cryo-electron Tomography of Influenza Viruses

Subnanometer-resolution Structural Determination of Hemagglutinin from Cryo-electron Tomography of Influenza Viruses

Published on: November 7, 2025

Real-time cryo-electron microscopy data preprocessing with Warp.

Dimitry Tegunov¹, Patrick Cramer²

¹Max Planck Institute for Biophysical Chemistry, Department of Molecular Biology, Göttingen, Germany. dteguno@mpibpc.mpg.de.

|October 9, 2019

Summary

This summary is machine-generated.

Warp software automates cryo-electron microscopy (cryo-EM) data preprocessing for enhanced quality and efficiency. This tool improves data analysis, leading to higher resolution cryo-EM structures.

More Related Videos

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Published on: May 29, 2021

Related Experiment Videos

Last Updated: Jan 6, 2026

Subnanometer-resolution Structural Determination of Hemagglutinin from Cryo-electron Tomography of Influenza Viruses

Subnanometer-resolution Structural Determination of Hemagglutinin from Cryo-electron Tomography of Influenza Viruses

Published on: November 7, 2025

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Published on: May 29, 2021

Area of Science:

Structural Biology
Biophysics
Microscopy

Background:

Cryo-electron microscopy (cryo-EM) data acquisition requires robust preprocessing for optimal results.
Current preprocessing workflows can be time-consuming and complex, impacting data quality and microscope efficiency.

Purpose of the Study:

To introduce Warp, a novel software solution for automating cryo-EM data preprocessing.
To enable real-time evaluation of cryo-EM data quality during acquisition.

Main Methods:

Warp automates micrograph correction for global and local motion.
It estimates local defocus and monitors key acquisition parameters in real time.
Includes deep learning models for particle picking and image denoising.

Main Results:

Demonstrated significant improvement in nominal resolution from 3.9 Å to 3.2 Å for a cryo-EM dataset.
Warp facilitates seamless integration with existing cryo-EM data processing pipelines.
The software is computationally inexpensive and user-friendly.

Conclusions:

Warp streamlines and enhances the cryo-EM data acquisition and preprocessing workflow.
The software contributes to achieving higher resolution structural information from biological specimens.
Warp is readily accessible and efficient for the cryo-EM community.