A potential marker in brucellosis, long non coding RNA IFNG-AS1

Area of Science:

• Immunology
• Molecular Biology
• Infectious Diseases

Background:

• Brucellosis is a prevalent bacterial zoonotic infection with pathogen persistence.
• Understanding host immune responses is crucial for managing brucellosis.

Purpose of the Study:

• To investigate the relationship between long noncoding RNA IFNG-AS1 and interferon gamma (IFN-γ) in brucellosis patients.
• To evaluate IFNG-AS1 as a potential biomarker in different stages of brucellosis.

Main Methods:

• Quantitative polymerase chain reaction (qPCR) to measure LncRNA IFNG-AS1, T-bet, and IFN-γ expression in PBMCs.
• Enzyme-linked immunosorbent assay (ELISA) to assess serum IFN-γ levels.
• Comparison between 60 brucellosis patients (acute, chronic, relapse) and 20 controls.

Main Results:

• Significantly elevated LncRNA IFNG-AS1, T-bet, and IFN-γ expression in all brucellosis patient groups compared to controls.
• Higher serum IFN-γ levels observed in acute and relapsed brucellosis groups.
• No significant difference in T-bet expression between chronic brucellosis and healthy groups.

Conclusions:

• LncRNA IFNG-AS1 plays a role in the immune response to brucellosis by enhancing IFN-γ expression.
• LncRNA IFNG-AS1 presents a promising potential biomarker for brucellosis screening, diagnosis, and treatment.