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Published on: June 24, 2018
Analysis of Molecular Changes Induced By Mineral Trioxide Aggregate On sPLA2
Murilo B Lopes1, Veronica C G Soares2, Fabio H R Fagundes3
1Department of Restorative Dentistry, UNOPAR - Universidade Norte do Paraná, Londrina, PR, Brazil.
Abstract:
The aim of this study was to analyze the effects of MTA on the structure and enzymatic activity of sPLA2 in order to provide subsidies for improvement in the formulation of the product. MTA powder was incubated for 60 min in the presence of sPLA2 and was analyzed by chromatography, electrospray mass (ESI-MS) and small-angle X-ray scattering (SAXS). It was find that the elution profile, retention time, and fragmentation of sPLA2 were altered after treatment with MTA. Calcium was the MTA component that most amplified the inflammatory signal. Significant interactions were found between MTA and sPLA2, which could aid in our understanding of the mechanisms of action of MTA during the inflammatory process and it may facilitate the structural modification of MTA, thereby improving its biological safety and consequently the rate of the treatment success.
