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Updated: Jan 6, 2026

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

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Published on: October 8, 2021

Barcoding Biological Reactions with DNA-Functionalized Vesicles.

Justin A Peruzzi¹, Miranda L Jacobs², Timothy Q Vu²

¹Department of Chemical and Biological Engineering, Northwestern University, USA.

Angewandte Chemie (International Ed. in English)

|October 10, 2019

Summary

This summary is machine-generated.

DNA tethers and phase-segregated membranes enable targeted vesicle fusion for controlled biological reactions. This approach facilitates in vitro protein expression and advances artificial cellular systems.

Keywords:

artificial cell biophysics cell-free reactions membrane fusion vesicles

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Area of Science:

Biotechnology
Synthetic Biology
Biochemistry

Background:

Targeted vesicle fusion offers a method to control interactions between cellular compartments.
Initiating biological reactions in complex environments requires precise control over molecular interactions.

Purpose of the Study:

To investigate how DNA tethers and phase-segregated membranes enhance targeted vesicle fusion.
To demonstrate the utility of engineered vesicle fusion for initiating cell-free protein expression.

Main Methods:

Utilizing DNA tethers to direct fusion between specific vesicle populations.
Employing phase-segregated membranes to provide an energetic driving force for fusion.
Demonstrating in vitro protein expression using fused vesicles as a model system.

Main Results:

Membrane phase-segregation significantly increases the efficiency of DNA-mediated vesicle fusion.
Orthogonal DNA tethers enable specific fusion events and targeted DNA cargo delivery.
Successful initiation of in vitro protein expression for soluble and membrane proteins was achieved.

Conclusions:

Engineered vesicle fusion using DNA tethers and phase segregation provides a novel strategy for controlling spatiotemporal dynamics of cell-free reactions.
This approach expands the possibilities for engineering artificial cellular systems with precise control over biological processes.