Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ultrasound-Activatable Piezoelectric Hydrogel Reprograms Mitochondrial Epigenetics for Osteoarthritis Therapy via the mTOR/GATD3A Axis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Flexibly Reinforced Polycaprolactone Bioelectrodes for Piezoresistive Sensing via Direct Ink Writing.

Journal of biomedical materials research. Part B, Applied biomaterials·2025
Same author

Mechanically reinforced core-shell scaffold with integrated structure and function for accelerated tendon repair.

Regenerative biomaterials·2025
Same author

Silver, silicon co-substituted hydroxyapatite modulates bacteria-cell competition for enhanced osteogenic function.

Biomedical materials (Bristol, England)·2021
Same author

Functional regeneration of tendons using scaffolds with physical anisotropy engineered via microarchitectural manipulation.

Science advances·2018
Same author

Macrophages Undergo M1-to-M2 Transition in Adipose Tissue Regeneration in a Rat Tissue Engineering Model.

Artificial organs·2016

Related Experiment Video

Updated: Jan 6, 2026

A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation
06:57

A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation

Published on: August 5, 2018

9.3K

Scaffold with Micro/Macro-Architecture for Myocardial Alignment Engineering into Complex 3D Cell Patterns.

Wanqi Zhang1, Zuyong Wang1, Chao Xie2

  • 1College of Materials Science and Engineering, Hunan University, Changsha, 410082, P. R. China.

Advanced Healthcare Materials
|October 11, 2019
PubMed
Summary
This summary is machine-generated.

Engineered scaffolds with micro/macro-architecture guide human mesenchymal stem cell alignment for myocardial tissue engineering. This 3D construct mimics natural heart tissue structure, advancing regenerative medicine possibilities.

Keywords:
3Dcell alignmentmesenchymal stem cellsscaffoldstissue engineering

More Related Videos

Generation of Aligned Functional Myocardial Tissue Through Microcontact Printing
11:09

Generation of Aligned Functional Myocardial Tissue Through Microcontact Printing

Published on: March 19, 2013

11.5K
Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells
10:37

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

Published on: March 14, 2021

7.2K

Related Experiment Videos

Last Updated: Jan 6, 2026

A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation
06:57

A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation

Published on: August 5, 2018

9.3K
Generation of Aligned Functional Myocardial Tissue Through Microcontact Printing
11:09

Generation of Aligned Functional Myocardial Tissue Through Microcontact Printing

Published on: March 19, 2013

11.5K
Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells
10:37

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

Published on: March 14, 2021

7.2K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Heart function relies on complex tissue structural anisotropy.
  • Regenerating aligned heart tissue using macroscale 3D constructs remains challenging in myocardial tissue engineering.

Purpose of the Study:

  • To investigate the feasibility of engineered scaffolds with micro/macro-architecture for guiding spatial cell alignment in complex patterns.
  • To develop a 3D construct for myocardial tissue engineering that mimics natural heart tissue alignment.

Main Methods:

  • Fabrication of stackable dual-structured scaffold layers with linear micro-ridge/groove patterns and macro-through-hole arrays.
  • Seeding human mesenchymal stem cells onto the patterned scaffolds and nonpatterned controls.
  • Analyzing cell alignment, orientation, and spatial distribution within the scaffold architecture.

Main Results:

  • Scaffolds enabled tailorable anisotropy and interconnective free space.
  • Human mesenchymal stem cells demonstrated well-organized spreading alignment with precise orientation control on patterned scaffolds compared to controls.
  • Observed spatial cell distribution and directional changes in scaffold patterns led to 3D reconstruction of cellular alignment resembling natural myocardial tissue.

Conclusions:

  • Micro/macro-architecture engineering is a viable strategy for spatial cell guidance.
  • Engineered scaffolds with tailored micro/macro-architecture show potential for biomanufacturing structural alignment in myocardial tissue engineering.