Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

17.2K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
17.2K
Enzyme-linked Receptors01:00

Enzyme-linked Receptors

85.5K
Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
85.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dual-frequency fiber-array photoacoustic computed tomography for high-resolution deep brain imaging.

Light, science & applications·2026
Same author

All-fiber photoacoustic endomicroscopy reveals layer-specific angiogenesis-oxygenation uncoupling in experimental colitis.

Science advances·2026
Same author

Multifunctional fiber-optic theranostic probe for closed-loop tumor photothermal therapy.

Light, science & applications·2026
Same author

Phase-coherent multi-sensor synthesis for enhanced photoacoustic imaging: a comprehensive framework for optimal sensor integration.

Biomedical optics express·2026
Same author

A High-Sensitive Photothermal-Readout Strategy for Commercial Lateral Flow Immunoassays with an Optical Microfiber Mach-Zehnder Interferometer.

Analytical chemistry·2026
Same author

Dynamic microvascular monitoring with miniaturized omnidirectional broadband photoacoustic imaging system for living entities (MOBILE).

Nature communications·2026
Same journal

Heterojunction-Enhanced Interfacial Evanescent-Tunable Fiber Optic Probe for Amplification-free CRISPR/Cas12a-Based Rapid and Ultrasensitive Detection of MPXV.

Analytical chemistry·2026
Same journal

Tunable Charge Transfer in Europium Metal-Organic Frameworks for Ratiometric Sensing of a Sarin Simulant.

Analytical chemistry·2026
Same journal

A β-Cyclodextrin/Ag<sub>2</sub>O@MWCNT-Based Stochastic Platform for the Simultaneous Molecular Enantiorecognition and Enantioanalysis of Twelve Amino Acids in Biological Matrices.

Analytical chemistry·2026
Same journal

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026
Same journal

Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

Analytical chemistry·2026
Same journal

H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jan 6, 2026

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

10.1K

Optical Microfiber Reader for Enzyme-Linked Immunosorbent Assay.

Li-Peng Sun1, Yan Huang1, Tiansheng Huang1

  • 1Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communication, Institute of Photonics Technology , Jinan University , Guangzhou 511443 , China.

Analytical Chemistry
|October 11, 2019
PubMed
Summary
This summary is machine-generated.

This study introduces an optical microfiber reader for highly sensitive detection of disease biomarkers using enzyme-linked immunosorbent assays (ELISA). The technology achieves picogram-level detection of C-reactive protein (CRP) and interleukin-6 (IL-6), improving early disease diagnosis.

More Related Videos

Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays
09:04

Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays

Published on: October 21, 2016

15.1K
Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

2.0K

Related Experiment Videos

Last Updated: Jan 6, 2026

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

10.1K
Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays
09:04

Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays

Published on: October 21, 2016

15.1K
Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

2.0K

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Optical Biosensing

Background:

  • Accurate detection of disease biomarkers at ultralow concentrations is crucial for timely clinical diagnosis and improved patient outcomes.
  • Current detection methods may lack the sensitivity required for early-stage disease identification.

Purpose of the Study:

  • To develop and validate an optical microfiber reader for enhanced sensitivity in enzyme-linked immunosorbent assays (ELISA).
  • To demonstrate the capability of the device for detecting disease biomarkers like C-reactive protein (CRP) and interleukin-6 (IL-6) at low concentrations.

Main Methods:

  • An optical microfiber reader was utilized to detect changes in refractive index (RI) caused by enzymatic reactions in ELISA.
  • The reader was applied to C-reactive protein (CRP) and interleukin-6 (IL-6) assays following enzymatic signal amplification.
  • Detection limits were assessed in phosphate-buffered saline (PBS) and human serum.

Main Results:

  • Log-linear relationships were established between biomarker concentration and spectral response for both CRP and IL-6 at nanogram and picogram levels.
  • Detection of anti-CRP/HRP as low as 9.75 pg/mL was achieved, surpassing conventional spectrophotometry sensitivity.
  • The system demonstrated stable, accurate, and color-free detection.

Conclusions:

  • The optical microfiber reader offers enhanced analytical sensitivity for ELISA, enabling detection of biomarkers at picogram levels.
  • This technology shows significant promise for early disease diagnosis and clinical monitoring.
  • The device provides a stable and accurate alternative to conventional detection methods.