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Updated: Jan 6, 2026
Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial Cysts
Published on: May 16, 2020
Ultrastructural Characteristics of Gallbladder Epithelial Inclusions Mimicking Cystoisospora
Arun Gopinath1, Aysha Mubeen1, Brett Baskovich1
1Department of Pathology, University of Florida Health, College of Medicine, Jacksonville.
Objectives:
There is recently reported increased prevalence of Isospora organisms in cholecystectomy specimens from immunocompetent patients, especially in acalculous cholecystectomies. We performed an ultrastructural and molecular evaluation of these specimens.
Methods:
From 28 gallbladders with intraepithelial inclusions, two specimens with diffuse involvement of the gallbladder epithelium were analyzed by electron microscopy. Polymerase chain reaction was performed on five samples for the ITS2 region of C belli and eukaryotic 18S region. The 18S products were sequenced by next-generation sequencing.
Results:
Electron microscopic analysis showed cytoplasmic condensations leading to vacuole formation. In contrast with true C belli, there were no identifiable organelles or organization. None of these cases showed amplified products other than human on molecular analysis.
Conclusions:
Electron microscopic analysis demonstrates that the inclusions are condensed cytoplasmic material and not true organisms.
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