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Calculating the Tumor Nuclei Content for Comprehensive Cancer Panel Testing.

Masashi Mikubo¹, Katsutoshi Seto¹, Atsuko Kitamura²

¹Department of Pathology and Molecular Diagnostics, Aichi Cancer Center, Nagoya, Japan.

Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer

|October 13, 2019

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Pathologists often overestimate tumor cell content (%TN) in tissue samples. Focused training significantly improved their accuracy in estimating %TN for comprehensive genetic panel testing.

Area of Science:

Oncology
Pathology
Genetics

Background:

Comprehensive genetic panel testing requires high tumor nuclei content (%TN) for accurate results.
Pathologists are critical for selecting appropriate tissue specimens and estimating %TN.

Purpose of the Study:

To evaluate intraobserver variability in %TN estimation by pathologists.
To determine if focused training and feedback can enhance pathologist accuracy in %TN assessment.

Main Methods:

Nine pathologists estimated %TN in 18 tumor specimens before and after training.
Estimates were compared against standards from a sequencing institution's pathologist and computational analysis.

Main Results:

Pathologists initially overestimated %TN, showing significant differences from standard values.

Keywords:

Comprehensive genetic panel testing FoundationOne CDx Molecular testing Pathological evaluation Tumor nuclei content

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Post-training, pathologist %TN estimates became significantly concordant with the standards.

Conclusions:

Empirical overestimation of %TN by pathologists is common.
Training and feedback programs can substantially improve pathologist accuracy in %TN evaluation for genetic testing.