Investigating the reproducibility of maximal oxygen uptake responses to high-intensity interval training

To test the hypothesis that observed maximal oxygen uptake (VO₂max) and time to fatigue (TTF) responses to two identical periods of standardized high-intensity interval training are reproducible.

Fourteen recreationally active and healthy young males completed two identical four-week periods of high-intensity interval training (4×4-min intervals at 90-95% maximum heart rate [HR_max] separated by 3-min periods of active recovery at 70-75% HR_max). Training periods were separated by a three-month washout period.

VO₂max and TTF were assessed via incremental tests with supramaximal verification before and after each training period. Pearson correlation coefficients (r), intraclass correlation coefficients (ICC), and within-subjects coefficients of variation (CV) were used to assess reproducibility of observed VO₂max and TTF responses.

VO₂max and TTF values before the second training period were not significantly higher than baseline values and there were no significant (p>0.05) interaction effects (period 1: VO₂max: +4.04±2.29mL/kg/min, TTF: +70.75±35.87s; period 2: VO₂max: +2.83±2.74mL/kg/min, TTF: +83.46±34.55s). We found very weak-to-moderate correlations and poor reproducibility for observed VO₂max (mL/kg/min: r=0.40, ICC=0.369, CV=74.4) and TTF (r=0.11. ICC=0.048, CV=45.6) responses to training periods 1 and 2.

Our ANOVA results confirmed that the three-month washout period returned VO₂max and TTF levels to baseline and prevented carryover effects. Contrary to our hypothesis, our results suggest that individual observed VO₂max and TTF responses to identical training stimuli are not reproducible.