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Updated: Jan 5, 2026

Author Spotlight: Advancements in Correlative Light and Electron Microscopy with Fluorescent Protein Preservation
Published on: January 12, 2024
mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM
Zhifei Fu1,2, Dingming Peng1,2, Mingshu Zhang3
1Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
Abstract:
Super-resolution correlative light and electron microscopy (SR-CLEM) is a powerful approach for imaging specific molecules at the nanoscale in the context of the cellular ultrastructure. Epon epoxy resin embedding offers advantages for SR-CLEM, including ultrastructural preservation and high quality sectioning. However, Epon embedding eliminates fluorescence from most fluorescent proteins. We describe a photocontrollable fluorescent protein, mEosEM, that can survive Epon embedding after osmium tetroxide (OsO4) treatment for improved SR-CLEM.
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