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Updated: Jan 5, 2026

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
Published on: November 8, 2019
"Off-on" switching of intracellular singlet oxygen release under biocompatible conditions
Esma Ucar1, Dongmei Xi, Ozlem Seven
1State Key Laboratory of Fine Chemicals, Department of Pharmacy, Dalian University of Technology, 2 Linggong Road, 116024, Dalian, China. sunwen@dlut.edu.cn eua@dlut.edu.cn.
Abstract:
Precise spatiotemporal control of singlet oxygen generation is of immense importance considering its involvement in photodynamic therapy. In this work, we present a rational design for an endoperoxide which is highly stable at ambient temperatures yet, can rapidly be converted into a highly labile endoperoxide, thus releasing the "stored" singlet oxygen on demand. The "off-on" chemical switching from the stable to the labile form is accomplished by the reaction with fluoride ions. The potential utility of controlled singlet oxygen release was demonstrated in cell cultures.
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