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Published on: November 16, 2017
Comparison of Microneutralization and Histo-Blood Group Antigen-Blocking Assays for Functional Norovirus Antibody
Robert L Atmar1,2, Khalil Ettayebi2, B Vijayalakshmi Ayyar2
1Department of Medicine, Baylor College of Medicine, Houston, Texas, USA.
Background:
The development of an in vitro cultivation system for human noroviruses allows the measurement of neutralizing antibody levels.
Methods:
Serum neutralizing antibody levels were determined using a GII.4/Sydney/2012-like virus in human intestinal enteroids in samples collected before and 4 weeks after administration of an investigational norovirus vaccine and were compared with those measured in histo-blood group antigen (HBGA)-blocking assays.
Results:
Neutralizing antibody seroresponses were observed in 71% of 24 vaccinated adults, and antibody levels were highly correlated (r = 0.82, P < .001) with those measured by HBGA blocking.
Conclusions:
HBGA-blocking antibodies are a surrogate for neutralization in human noroviruses.
Clinical Trials Registration:
NCT02475278.
