Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Gene Therapy

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...

Microorganisms in Medicine and Therapeutics

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

IDH1-R132H enhances oncolytic HSV-1 therapy by facilitating viral entry and immune activation in glioma.

Nature communications·2026

Same author

NMR insights into the role of urea in cellulose dissolution: A stoichiometric study of cellobiose in NaOH/urea aqueous solution.

Magnetic resonance letters·2026

Same author

Lithio-Gel via Lithium Bonding: Mitigating Anode Failure by Blocking Crosstalk in Rechargeable Li-SOCl<sub>2</sub> Batteries.

Journal of the American Chemical Society·2026

Same author

Direct Visual Chiral Discrimination of Lys and Arg Enantiomers by the Achiral MIL-101(Cr)-NH<sub>2</sub> Platform.

Analytical chemistry·2026

Same author

Controllable Preparation and Performance Investigation of Amino Acid Functionalized Mixed-Mode Chromatographic Stationary Phase.

Precision chemistry·2026

Same author

Correction: Genetic mutations in HSV-1 replication-defective vectors: Implications for their safety in gene therapy applications.

Gene therapy·2026

Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 5, 2026

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Published on: January 7, 2019

Engineering HSV-1 Vectors for Gene Therapy.

William F Goins¹, Shaohua Huang², Bonnie Hall²

¹Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. goins@pitt.edu.

Methods in Molecular Biology (Clifton, N.J.)

|October 17, 2019

Summary

This summary is machine-generated.

Gene therapy using herpes simplex virus (HSV) vectors is advancing patient care for brain cancer and melanoma. Researchers are developing improved replication-defective HSV vectors for neurological disorders and long-term gene expression.

Keywords:

Gene therapy Gene transfer Herpes simplex virus Virus production Virus purification Virus vectors

More Related Videos

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Published on: January 7, 2019

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

Published on: March 10, 2023

Related Experiment Videos

Last Updated: Jan 5, 2026

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Published on: January 7, 2019

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Published on: January 7, 2019

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

Published on: March 10, 2023

Area of Science:

Biomedical Engineering
Gene Therapy
Oncolytic Viruses

Background:

Gene therapy has become standard patient care with approved products like Glybera.
Replication-competent herpes simplex virus (HSV) vectors are used in clinical trials for glioblastoma multiforme (GBM) and malignant melanoma.
Imlygic® (T-VEC) is an FDA-approved oncolytic HSV vector for melanoma, showing enhanced efficacy when combined with checkpoint inhibitors.

Purpose of the Study:

To describe methods for constructing recombinant genomic HSV vectors.
To detail purification steps for these vectors.
To outline small-scale production for cell culture and preclinical studies.

Main Methods:

Construction of recombinant genomic HSV vectors using HSV-1 replication-defective backbones.
Purification of engineered HSV vectors.
Small-scale production of vectors for experimental use.

Main Results:

Development of replication-defective, nontoxic HSV vectors with long-term transgene expression in neurons.
Demonstrated efficacy of oncolytic HSV vectors like T-VEC in clinical trials for cancer.
Successful combination therapy of Imlygic® with checkpoint inhibitors for melanoma.

Conclusions:

Replication-defective HSV vectors are promising for treating neurological disorders.
Engineered HSV vectors offer a platform for advanced gene therapy applications.
The described methods facilitate the use of HSV vectors in research and preclinical development.