Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

2.2K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.2K
Catalysis02:50

Catalysis

30.0K
The presence of a catalyst affects the rate of a chemical reaction. A catalyst is a substance that can increase the reaction rate without being consumed during the process. A basic comprehension of a catalysts’ role during chemical reactions can be understood from the concept of reaction mechanisms and energy diagrams.
30.0K
Solvents01:12

Solvents

69.4K
A solvent is a substance, most often a liquid, that can dissolve other substances. Here, the substance being dissolved is called a solute. When a solvent and a solute combine, they form a solution - a homogenous mixture of both the solvent and the solute. Water is a universal biological solvent. Its polar structure allows it to dissolve many other polar compounds. The ability of water to dissolve is governed by a balance between water molecules binding to each other and binding to the solute.
A...
69.4K
Reduction of Alkenes: Asymmetric Catalytic Hydrogenation02:17

Reduction of Alkenes: Asymmetric Catalytic Hydrogenation

3.8K
Catalytic hydrogenation of alkenes is a transition-metal catalyzed reduction of the double bond using molecular hydrogen to give alkanes. The mode of hydrogen addition follows syn stereochemistry.
The metal catalyst used can be either heterogeneous or homogeneous. When hydrogenation of an alkene generates a chiral center, a pair of enantiomeric products is expected to form. However, an enantiomeric excess of one of the products can be facilitated using an enantioselective reaction or an...
3.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Oppositely Charged Single Enzyme Nanogels Form Versatile Coacervates for Efficient Enzyme Cascade Catalysis.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Light-tunable DNA interactions enable spatiotemporal assembly and relaxation-driven crystallization of colloids.

Soft matter·2026
Same author

Mechanically Activated Luminescence in Polyurethanes Incorporating Calixarene Mechanophores.

Angewandte Chemie (International ed. in English)·2026
Same author

Light and Enzymatic Cooperative Response in Supramolecular Fibers: A Synergistic Strategy for Potential Drug Delivery Applications.

Biomacromolecules·2026
Same author

Thermoresponsive Gels Based on Cross-Linked Polymer-Grafted Cellulose Nanocrystals.

Biomacromolecules·2026
Same author

Activity of ice-binding proteins can be markedly enhanced by protein tags.

Nanoscale·2026

Related Experiment Video

Updated: Jan 5, 2026

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes
09:42

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes

Published on: January 16, 2016

9.3K

Tandem catalysis in multicomponent solvent-free biofluids.

Dylan Luke Atkins1, José Augusto Berrocal2, Alexander Francesco Mason3

  • 1Laboratory of Self-Organizing Soft Matter, Department of Chemical Engineering and Chemistry, Eindhoven University of Technology, 5600 MB Eindhoven, The Netherlands. d.atkins@tue.nl i.voets@tue.nl and Laboratory of Physical Chemistry, Department of Chemical Engineering and Chemistry, Eindhoven University of Technology, 5600 MB Eindhoven, The Netherlands and Institute for Complex Molecular Systems, Department of Chemical Engineering and Chemistry, Eindhoven University of Technology, 5600 MB Eindhoven, The Netherlands.

Nanoscale
|October 18, 2019
PubMed
Summary

This study demonstrates a novel method using chemical modification and nanoencapsulation to create stable enzymes. These engineered biocatalysts exhibit extreme stability, functioning at high temperatures and low hydration levels.

More Related Videos

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
14:43

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

11.2K
Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells
11:30

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

Published on: January 26, 2017

15.5K

Related Experiment Videos

Last Updated: Jan 5, 2026

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes
09:42

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes

Published on: January 16, 2016

9.3K
Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
14:43

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

11.2K
Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells
11:30

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

Published on: January 26, 2017

15.5K

Area of Science:

  • Biocatalysis and Enzyme Engineering
  • Materials Science
  • Chemical Industry Applications

Background:

  • Enzymes are crucial biocatalysts in chemical industries, offering selectivity and efficiency under mild conditions.
  • Poor enzyme stability under harsh reaction conditions limits their industrial application.
  • Current solutions like protein engineering or immobilization can reduce enzyme activity or specificity.

Purpose of the Study:

  • To develop a method for enhancing enzyme stability and performance under extreme conditions.
  • To create robust biocatalysts for challenging chemical transformations.
  • To overcome the limitations of traditional enzyme stabilization techniques.

Main Methods:

  • Utilizing a combinatorial approach of chemical modification and supramolecular nanoencapsulation.
  • Developing a protein-polymer surfactant core-shell architecture for enzyme stabilization.
  • Investigating enzyme behavior at low hydration levels and high temperatures.

Main Results:

  • Enzymes were endowed with apparent extremophilic behavior through chemical modification and nanoencapsulation.
  • The protein-polymer surfactant architecture allowed enzyme activity at hydration levels significantly below solvation.
  • Constructed biofluids demonstrated tandem operation at temperatures up to 150 °C without solvent.

Conclusions:

  • A viscous surfactant milieu can effectively replace both hydration and bulk water for enzyme function.
  • This approach successfully promotes extremophilic traits in enzymes with diverse catalytic functions.
  • The developed biofluids offer a promising strategy for industrial biocatalysis under demanding conditions.