Saliniradius amylolyticus gen. nov., sp. nov., isolated from solar saltern sediment

A novel non-pigmented, Gram-stain-negative, motile by means of a polar flagellum, aerobic and rod-shaped bacterium, designated HMF8227^T, was isolated from solar saltern sediment sampled at Shinan, Republic of Korea. The isolate was able to grow at 15-42 °C (optimum, 37 °C), at pH 6-8 (pH 7) and with 0.5-12 % NaCl (2-5 %). Strain HMF8227^T was positive for hydrolysis of starch and dextrin. 16S rRNA gene sequence analysis revealed that strain HMF8227^T was affiliated with the family Alteromonadaceae, sharing the highest sequence similarities to the genera Salinimonas (93.0-94.4 %), Aestuariibacter (92.0-94.2 %), Alteromonas (92.0-93.6 %) and Lacimicrobium (93.6 %). In the phylogenetic trees, strain HMF8227^T formed an independent clade with Lacimicrobium alkaliphilum X13M-12^T. The major fatty acids were C_16 : 0, summed feature 3 (C_16 : 1 ω7c and/or C_16 : 1 ω6c) and summed feature 8 (C_18 : 1 ω7c and/or C_18 : 1 ω6c). The major respiratory quinone was ubiquinone-8 (Q-8). The major polar lipids are phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid and two unidentified glycolipids. The DNA G+C content of the genomic DNA was 52.1 mol%. On the basis of the polyphasic characterizations, strain HMF8227^T represents a novel species and genus within the family Alteromonadaceae, for which the name Saliniradius amylolyticus gen. nov., sp. nov. is proposed, with the type strain being HMF8227^T (=KCTC 62462^T =NBRC 113230^T).