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The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

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Enhancing Tumor Content through Tumor Macrodissection

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STAT3, tumor microenvironment, and microvessel density in diffuse large B cell lymphomas.

Roberto Tamma¹, Giuseppe Ingravallo², Francesco Gaudio³

¹Department of Basic Medical Sciences, Neurosciences, and Sensory Organs, University of Bari Medical School, Bari, Italy.

Leukemia & Lymphoma

|October 19, 2019

Summary

Activated STAT3 in diffuse large B-cell lymphoma (DLBCL) correlates with poor survival. In activated B-cell (ABC) DLBCL, higher STAT3 expression is linked to increased inflammatory cells, angiogenesis, and tumor cell proliferation.

Keywords:

DLBCL lymphomas RNAscope STAT3 lymphocytes tumor-associated macrophages

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Published on: June 7, 2018

Area of Science:

Oncology
Immunology
Pathology

Background:

Constitutively activated STAT3 is linked to advanced clinical stage and poor survival in diffuse large B-cell lymphoma (DLBCL).
Understanding the tumor microenvironment in different DLBCL subtypes is crucial for therapeutic strategies.

Purpose of the Study:

To evaluate STAT3 and Ki67 expression, inflammatory cell infiltration, and microvascular density in DLBCL bioptic specimens.
To compare these markers between activated B-cell (ABC) and germinal center B (GCB) DLBCL subtypes.

Main Methods:

RNA-scope was used to quantify STAT3+ cells.
Immunohistochemistry was employed to assess CD3, CD8, CD68, CD163, CD34, and Ki67 positive cells.
Correlations between markers were analyzed in ABC and GCB groups.

Main Results:

ABC-DLBCL samples showed significantly higher STAT3+ cell counts compared to GCB-DLBCL.
Increased infiltration of CD3, CD8, CD68, CD163, CD34, and Ki67 positive cells was observed in ABC patients.
Positive correlations were found between STAT3 and inflammatory markers (CD3, CD8, CD68, CD163) in ABC-DLBCL.
STAT3 expression correlated with tumor cell proliferation (Ki67) and angiogenesis (CD34) in ABC-DLBCL.

Conclusions:

Higher STAT3 expression in ABC-DLBCL is associated with increased infiltration of tumor-associated macrophages (TAMs) and cytotoxic T cells.
These findings suggest a role for STAT3 in promoting an inflammatory and angiogenic tumor microenvironment in ABC-DLBCL.
STAT3 may represent a therapeutic target in DLBCL, particularly in the ABC subtype.