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Induced Intermediate Mesoderm Combined with Decellularized Kidney Scaffolds for Functional Engineering Kidney
Jianye Zhang1, Kailin Li2, Feng Kong2,3,4
11Department of Urology, The Second Hospital, Shandong University, 247 Beiyuan Street, Jinan, 250033 Shandong People's Republic of China.
Tissue Engineering and Regenerative Medicine
|October 19, 2019
Summary
Researchers induced intermediate mesoderm cells from adipose-derived stem cells (ADSCs) and showed they effectively differentiate within decellularized kidney scaffolds for potential kidney regeneration. This advances stem cell therapy for kidney repair.
Area of Science:
- Regenerative Medicine
- Stem Cell Biology
- Biomaterials Science
Background:
- Chronic kidney disease (CKD) poses a significant health burden with limited treatment options.
- Mammalian kidneys have a fixed nephron number, hindering natural regeneration after damage.
- Current strategies combining decellularized scaffolds and stem cells show insufficient stem cell differentiation for functional regeneration.
Purpose of the Study:
- To investigate the potential of induced intermediate mesoderm cells derived from adipose-derived stem cells (ADSCs) for kidney regeneration.
- To evaluate the differentiation efficiency of these induced cells within decellularized kidney scaffolds.
Main Methods:
- Rat kidneys were decellularized using 0.5% sodium dodecyl sulfate (SDS).
- Adipose-derived stem cells (ADSCs) were induced into intermediate mesoderm cells in vitro using CHIR99021 and FGF9.
- Decellularized scaffolds were recellularized with ADSCs and induced intermediate mesoderm cells via renal artery and ureter, followed by 10-day co-culture.
Main Results:
- Intermediate mesoderm cells were successfully induced from ADSCs, confirmed by OSR1 and PAX2 expression.
- Co-culture within decellularized scaffolds promoted differentiation of intermediate mesoderm cells into tubular-like (E-CAD+, GATA3+) and podocyte-like (WT1+) cells.
- Differentiation efficiency was higher in scaffolds compared to in vitro cultures of induced cells.
Conclusions:
- Adipose-derived stem cells can be induced into intermediate mesoderm cells.
- These induced cells exhibit enhanced differentiation within decellularized kidney scaffolds, showing promise for kidney regeneration strategies.
