Assessing Antibody Specificity in Human Serum Using Deep Sequence-Coupled Biopanning.
Kathryn M Frietze1,2, Susan B Core3, Alexandria Linville3
1Department of Molecular Genetics and Microbiology, School of Medicine, University of New Mexico Health Sciences, Albuquerque, NM, USA. kfrietze@salud.unm.edu.
Methods in Molecular Biology (Clifton, N.J.)
|October 19, 2019
Summary
This study presents a phage-display method to analyze antibody specificity in human serum. This technique aids in identifying peptide epitopes recognized by monoclonal antibodies for broader diagnostic applications.
Area of Science:
- Immunology
- Biotechnology
- Virology
Background:
- Phage-display technology is crucial for identifying monoclonal antibody epitopes.
- Deep sequencing enhances the analysis of selected phage populations.
Purpose of the Study:
- To describe a novel phage-display method for assessing antibody specificity in human serum.
- To adapt this method for various phage-display platforms.
Main Methods:
- Utilized a phage-display platform for affinity selection.
- Employed deep sequencing for comprehensive analysis of selectants.
- Demonstrated the method using the bacteriophage MS2 virus-like particle (VLP) platform.
Main Results:
- Successfully established a method to evaluate antibody specificity against peptide epitopes in serum.
- The described method is adaptable to different phage-display systems.
Conclusions:
- Phage-display coupled with deep sequencing offers a powerful approach for antibody specificity profiling.
- This method has potential applications in diagnostics and therapeutic antibody development.
