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Updated: Jan 5, 2026

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
Published on: June 23, 2022
Catalyst-proximity protein chemical labelling on affinity beads targeting endogenous lectins
Michihiko Tsushima1, Shinichi Sato2, Tatsuya Niwa3
1Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, 4259-R1-13, Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8503, Japan. shinichi.sato@res.titech.ac.jp hiro@res.titech.ac.jp and School of Life Science and Engineering, Tokyo Institute of Technology, Japan.
Abstract:
Magnetic affinity beads functionalized with lactose and ruthenium/dcbpy complexes were developed. Using MAUra, a catalyst-proximity labelling reagent, the catalytic labeling of lactose-binding proteins was achieved with high selectivity on the beads. The first unbiased identification of cellular endogenous lectins bound to lactose (galectin-1 and galectin-3) was achieved with chemical labelling on the affinity beads.
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