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Published on: April 18, 2019
Chemical Modification of Microcin J25 Reveals New Insights on the Stereospecific Requirements for Antimicrobial
Helena Martin-Gómez1, Marta Jorba2, Fernando Albericio3,4,5
1Institute for Research in Biomedicine, Baldiri Reixac 10, 08028 Barcelona, Spain. marting.helena@gmail.com.
Abstract:
In this study, microcin J25, a potent antimicrobial lasso peptide that acts on Gram-negative bacteria, was subjected to a harsh treatment with a base in order to interrogate its stability and mechanism of action and explore its structure-activity relationship. Despite the high stability reported for this lasso peptide, the chemical treatment led to the detection of a new product. Structural studies revealed that this product retained the lasso topology, but showed no antimicrobial activity due to the epimerization of a key residue for the activity. Further microbiological assays also demonstrated that it showed a high synergistic effect with colistin.
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