Jove
Visualize
Contact Us

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.8K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Individualized decision-making driven by long-term memory retrieval in Drosophila.

Cell reports·2026
Same author

Self-Supervised Isotropic Resolution Enhancement of Expansion Microscopy via Quantized Compression.

Journal of imaging informatics in medicine·2026
Same author

A modular tissue-clearing framework integrated with light-field microscopy enables rapid volumetric phenotyping of cardiac tissue.

Research square·2026
Same author

Redox regulation of memory formation by Rrp1 in <i>Drosophila</i>.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Proximity-induced nodal metal in an extremely underdoped CuO<sub>2</sub> plane in triple-layer cuprates.

Nature communications·2025
Same author

Examining the effects of upward social mobility on women's workplace experiences in China.

Frontiers in psychology·2025
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jan 5, 2026

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
08:32

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

3.1K

Rapid single-wavelength lightsheet localization microscopy for clarified tissue.

Li-An Chu1,2, Chieh-Han Lu2,3,4, Shun-Min Yang2

  • 1Brain Research Center, National Tsing Hua University, 101, Section 2, Kuang-Fu Road, Hsinchu, 30013, Taiwan.

Nature Communications
|October 20, 2019
PubMed
Summary

We developed a new super-resolution microscopy technique for imaging entire animal organs at the nanoscale. This method enables fast, high-resolution imaging of neural structures in whole Drosophila brains, revealing protein distribution.

More Related Videos

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart
08:42

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart

Published on: September 15, 2018

9.7K
Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos
10:15

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

Published on: April 28, 2017

11.0K

Related Experiment Videos

Last Updated: Jan 5, 2026

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
08:32

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

3.1K
Light-sheet Fluorescence Microscopy for the Study of the Murine Heart
08:42

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart

Published on: September 15, 2018

9.7K
Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos
10:15

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

Published on: April 28, 2017

11.0K

Area of Science:

  • Biophysics
  • Neuroscience
  • Microscopy

Background:

  • Optical super-resolution microscopy enables nanoscale protein imaging in biological tissues.
  • Large-volume super-resolution imaging of entire animal organs remains a significant challenge due to optical aberrations in thick tissues.

Purpose of the Study:

  • To develop an advanced super-resolution microscopy method for large-volume imaging of intact biological specimens.
  • To overcome limitations in imaging thick tissues and achieve nanoscale resolution across entire organs.

Main Methods:

  • Development of a single-wavelength Bessel lightsheet microscopy technique.
  • Optimization for refractive-index matching with clarified specimens to minimize aberrations.
  • Utilizing spontaneous blinking fluorophores for protein labeling and quantitative single-molecule localization.

Main Results:

  • Achieved nanoscale imaging of nearly all dopaminergic neurons in the whole adult Drosophila melanogaster brain (3.64 × 10^7 µm³).
  • Demonstrated high imaging speed (436 µm³/second) for localization in large volumes.
  • Resolved subcellular protein distribution of a monoamine transporter in a specific serotonergic neuron (DPM).

Conclusions:

  • The developed Bessel lightsheet method enables unprecedented large-volume super-resolution imaging of neural architecture.
  • This technique provides robust statistical analysis of nanoscale data from entire organs, advancing neuroscience research.
  • Facilitates detailed investigation of protein localization and neuronal morphology in complex biological systems.