Digital PCR - Methods and Protocols
1Center for the Study of Myelofibrosis, Laboratory of Biochemistry, Biotechnology and Advanced Diagnosis, IRCCS Policlinico San Matteo Foundation, Pavia. p.catarsi@smatteo.pv.it.
European Journal of Histochemistry : EJH
|October 22, 2019
Abstract:
Sensitivity, reproducibility, precision and accuracy are recurring words in this book. Indeed, digital PCR (dPCR) represents a major step forward in the quantification of nucleic acids. In this field, for many years it has been used the quantitative PCR (qPCR), a technology that allows a real-time monitoring of DNA amplification which has proved useful for obtaining a relative measurement, but that only indirectly, through the use of reference standards, provides an absolute quantification....
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