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Related Experiment Video

Updated: Jan 5, 2026

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Published on: September 8, 2023

Angular-dependent polarization-based plasmon light scattering for bioaffinity sensing.

Kadir Aslan¹, Joseph R Lakowicz², Chris D Geddes³

¹Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, Maryland 21201.

Applied Physics Letters

|October 22, 2019

Summary

This summary is machine-generated.

This study introduces a new affinity biosensing method using gold nanoparticles. The technique detects molecular binding by measuring changes in light scattering polarization, enabling sensitive detection.

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Last Updated: Jan 5, 2026

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Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Published on: July 21, 2018

Area of Science:

Nanotechnology
Biophysics
Analytical Chemistry

Background:

Plasmon scattering from nanoparticles is sensitive to aggregation.
Biotin-streptavidin interactions are widely used in biosensing.
Polarization of scattered light can provide unique information about nanoparticle assemblies.

Purpose of the Study:

To develop a novel affinity biosensing approach using plasmon scattering.
To investigate the changes in light polarization upon nanoparticle aggregation induced by specific binding.
To establish a sensitive detection method based on optical properties of gold colloids.

Main Methods:

Coating 20 nm gold colloids with biotinylated-bovine serum albumin.
Inducing nanoparticle aggregation via streptavidin crosslinking.
Measuring the polarization and scatter of incident light at various angles.
Analyzing the relationship between binding events and optical signal changes.

Main Results:

Initial nanoparticles exhibited high polarization (P≈1) in a Rayleigh-like scattering manner.
Aggregation led to decreased polarization and increased forward scatter.
Significant polarization changes were observed at angles near 180°.
The observed phenomena are consistent with plasmon near-field coupling and Mie-like scattering.

Conclusions:

The described approach offers a sensitive method for affinity biosensing.
Changes in plasmon scatter polarization serve as a reliable indicator of molecular binding.
This technique leverages optical properties of gold nanoparticles for label-free detection.